{"id":28233,"date":"2020-08-29T10:33:40","date_gmt":"2020-08-29T10:33:40","guid":{"rendered":"https:\/\/poliklinika-analiza.hr\/product\/hdl-cholesterol\/"},"modified":"2026-06-12T23:14:53","modified_gmt":"2026-06-12T23:14:53","slug":"hdl-cholesterol","status":"publish","type":"product","link":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/","title":{"rendered":"HDL cholesterol"},"content":{"rendered":"<p><strong>DESCRIPTION:<\/strong> Cholesterol is distributed in all lipoprotein fractions. Cholesterol found in HDL-lipoprotein is considered to have a protective effect and to protect against coronary disease. HDL-cholesterol is synthesized in the liver and is released from the liver as a discoid particle that binds cholesterol in the form of cholesterol-ester, which is formed by the catalytic action of LCAT. In this way, &#8220;bad&#8221; cholesterol is removed from peripheral tissues (LDL-cholesterol). In the liver, this cholesterol is catabolized and excreted via bile as bile acid salts (Figure 22). About 20% of the total serum cholesterol is HDL-cholesterol (about 1.2 mmol\/L). Cholesterol is found in HDL2 and HDL3. The protective effect is attributed to HDL2, since HDL2 contains more esterified cholesterol than HDL3. Composition and characteristics of high-density lipoproteins (HDL): HDL: Density 1,063 \u2013 1,210 HDL2: Density 1,063 \u2013 1,125 HDL3: Density 1,125 \u2013 1,210 HDL: Proteins 52 HDL2: Proteins 42 HDL3: Proteins 56 HDL: Triglycerides 6 HDL2: Triglycerides 5 HDL3: Triglycerides 3 HDL: Cholesterol esters 14 HDL2: Cholesterol Esters 17 HDL3: Cholesterol Esters 19 HDL: Free Cholesterol 3 HDL2: Free Cholesterol HDL3: Free Cholesterol HDL: Phospholipids 24 HDL2: Phospholipids 36 HDL3: Phospholipids 22 HDL: Unsterified Fatty Acids 1 HDL2: Unsterified Fatty Acids HDL3: Unsterified Fatty Acids <strong>DETERMINATION:<\/strong> <\/strong> Direct enzymatic HDL cholesterol determination method according to IFCC. Sample: Serum or plasma. (Do not use anticoagulants containing citrate). The stability of the sample is stored for 7 days at 2\u00b0C to 8\u00b0C. The principle of the reaction is as follows: Cholesterol esterase catalyzes the hydrolysis of cholesterol esters into free cholesterol and free fatty acids. In the presence of cholesterol oxidase, cholesterol is oxidized to delta-4-cholesten-3-one and hydrogen peroxide is formed. Phenol and 4-aminoantipyrine with hydrogen peroxide in the presence of peroxidase give the red colored product quinonimine. The intensity of staining is directly proportional to the concentration of cholesterol in the sample. <strong>CLINICAL SIGNIFICANCEJ<\/strong>: The main function of HDL (high-density lipoprotein) is to transport cholesterol from peripheral tissues to the liver. HDL particles are mostly synthesized in the liver, and to a lesser extent in intestinal cells. Nascent HDL particles contain phospholipids, cholesterol, apo E and apo A, and have a discoidal shape. In the circulation, receiving apoC and free cholesterol from other lipoproteins and non-fat cells, they assume a spherical shape. Determination of HDL-cholesterol (high density lipoproteins) is used in preventive diagnostics. The concentration of HDL-cholesterol in the blood is associated with the development of atherosclerotic diseases, primarily with coronary heart diseases and peripheral circulation disorders. The lower the concentration of HDL-cholesterol in the blood, the greater the risk of developing the disease. 1) Physiological variations of HDL-cholesterol in the serum: Physical training \/ HDL-cholesterol concentration is higher in women than in men \/ Drinking small amounts of alcohol increases HDL-cholesterol concentration by about 1%. 2) Pathological changes in HDL-cholesterol concentration A) Increased HDL-cholesterol concentration values in: Essential hypertension \/ Hypothyroidism \/ Insulin-dependent diabetes mellitus (IDDM) \/ Chronic active hepatitis \/ Nephrotic syndrome \/ Primary sclerosing cholangitis \/ von Willebrand&#8217;s disease \/ Liver failure B) Decreased HDL-cholesterol concentration values in: Affective disorders \/ Acute myocardial infarction \/ Coronary artery diseases \/ Celiac disease \/ Diabetes mellitus \/ Essential hypertension \/ Gout \/ Hyperthyroidism \/ Hypoalphalipoproteinemia \/ IgA nephropathy \/ Insulin-independent diabetes mellitus (NIDM) \/ Insulin-dependent diabetes mellitus (IDDM) \/ Kawasaki disease \/ Chronic renal failure \/ Quadriplegia \/ Nephrotic syndrome \/ Peripheral artery diseases \/ Polycystic ovarian diseases \/ Postmenopause \/ Rheumatoid arthritis \/ Rheumatic diseases \/ Septicemia \/ Acquired immunodeficiency syndrome \/ Stroke (e.g. cerebral, cardiac) <strong>RISK FACTORS:<\/strong> <strong>Decreased values<\/strong>: \u2013 Alcoholism \u2013 Angiography (heparin) \u2013 Citrates \u2013 Dehydroepiandrosterone \u2013 Diet rich in carbohydrates \u2013 Age \u2013 Estrogen\/progesterone therapy \u2013 Physical training \u2013 Fluorides \u2013 Starvation Body position \u2013 Fish oil \u2013 Vegetarian diet <strong>Elevated values:<\/strong> \u2013 If whole blood is stored in test tubes at room temperature for 3 days, the concentration of HDL-cholesterol in the serum can be higher by 6%. &#8211; Alcoholism &#8211; Capillary blood (Higher by about 4%) &#8211; After major surgical procedures, HDL-cholesterol measurement should be postponed for at least 3 months. \u2013 Oral contraceptives \u2013 Obesity \u2013 Members of the black race \u2013 Smoking \u2013 Physical effort (after long-term effort HDL-cholesterol concentration measurement should be postponed for 72 hours) DRUG EFFECTS:<\/strong> <strong>Decreased values:<\/strong> ascorbic acid <strong>RESULT:<\/strong> The reference interval depends on gender, age and method of work. The reference interval is displayed on each validated result.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>DESCRIPTION: Cholesterol is distributed in all lipoprotein fractions. Cholesterol found in HDL-lipoprotein is considered to have a protective effect and to protect against coronary disease. HDL-cholesterol is synthesized in the liver and is released from the liver as a discoid particle that binds cholesterol in the form of cholesterol-ester, which is formed by the catalytic [&hellip;]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":[],"product_brand":[],"product_cat":[86],"product_tag":[],"class_list":["post-28233","product","type-product","status-publish","product_cat-tests","first","instock","virtual","purchasable","product-type-simple"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>HDL cholesterol - Poliklinika ANALIZA<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HDL cholesterol - Poliklinika ANALIZA\" \/>\n<meta property=\"og:description\" content=\"DESCRIPTION: Cholesterol is distributed in all lipoprotein fractions. Cholesterol found in HDL-lipoprotein is considered to have a protective effect and to protect against coronary disease. HDL-cholesterol is synthesized in the liver and is released from the liver as a discoid particle that binds cholesterol in the form of cholesterol-ester, which is formed by the catalytic [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/\" \/>\n<meta property=\"og:site_name\" content=\"Poliklinika ANALIZA\" \/>\n<meta property=\"article:modified_time\" content=\"2026-06-12T23:14:53+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/product\\\/hdl-cholesterol\\\/\",\"url\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/product\\\/hdl-cholesterol\\\/\",\"name\":\"HDL cholesterol - Poliklinika ANALIZA\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/#website\"},\"datePublished\":\"2020-08-29T10:33:40+00:00\",\"dateModified\":\"2026-06-12T23:14:53+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/product\\\/hdl-cholesterol\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/product\\\/hdl-cholesterol\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/product\\\/hdl-cholesterol\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Po\u010detna stranica\",\"item\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cjenik\",\"item\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/cjenik-sve-usluge\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"HDL cholesterol\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/\",\"name\":\"Poliklinika ANALIZA\",\"description\":\"Medicinsko-biokemijski laboratorij ANALIZA\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/poliklinika-analiza.hr\\\/en\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"HDL cholesterol - Poliklinika ANALIZA","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/","og_locale":"en_US","og_type":"article","og_title":"HDL cholesterol - Poliklinika ANALIZA","og_description":"DESCRIPTION: Cholesterol is distributed in all lipoprotein fractions. Cholesterol found in HDL-lipoprotein is considered to have a protective effect and to protect against coronary disease. HDL-cholesterol is synthesized in the liver and is released from the liver as a discoid particle that binds cholesterol in the form of cholesterol-ester, which is formed by the catalytic [&hellip;]","og_url":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/","og_site_name":"Poliklinika ANALIZA","article_modified_time":"2026-06-12T23:14:53+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/","url":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/","name":"HDL cholesterol - Poliklinika ANALIZA","isPartOf":{"@id":"https:\/\/poliklinika-analiza.hr\/en\/#website"},"datePublished":"2020-08-29T10:33:40+00:00","dateModified":"2026-06-12T23:14:53+00:00","breadcrumb":{"@id":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/poliklinika-analiza.hr\/en\/product\/hdl-cholesterol\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Po\u010detna stranica","item":"https:\/\/poliklinika-analiza.hr\/en\/"},{"@type":"ListItem","position":2,"name":"Cjenik","item":"https:\/\/poliklinika-analiza.hr\/cjenik-sve-usluge\/"},{"@type":"ListItem","position":3,"name":"HDL cholesterol"}]},{"@type":"WebSite","@id":"https:\/\/poliklinika-analiza.hr\/en\/#website","url":"https:\/\/poliklinika-analiza.hr\/en\/","name":"Poliklinika ANALIZA","description":"Medicinsko-biokemijski laboratorij ANALIZA","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/poliklinika-analiza.hr\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product\/28233","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/types\/product"}],"version-history":[{"count":2,"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product\/28233\/revisions"}],"predecessor-version":[{"id":28699,"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product\/28233\/revisions\/28699"}],"wp:attachment":[{"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/media?parent=28233"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product_brand?post=28233"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product_cat?post=28233"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/poliklinika-analiza.hr\/en\/wp-json\/wp\/v2\/product_tag?post=28233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}